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Quantifying Cholesterol Uptake by Macrophages

In this short Youtube video we demonstrate a simple method of measuring cholesterol uptake and intracellular transport in macrophages. In this experiment, macrophages are cultured in a media containing oxLDL (bad cholesterol) and a cholesterol/lipid-reactive dye (Nile red). Live cell time-lapse microscopy is then used to monitor the accumulation of cholesterol by the cell. The …

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More papers!

2015 has been a good year for the Heit lab, with five papers accepted for publication since January, with several other papers making their way through the review system. Our fifth paper of the year, a collaborative study with the Lee lab of St. Michaels Hospital in Toronto, has just been released in Cardiovascular Research. In this …

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Congratulations Masha!

On August 21st, 2015 Masha (Maria) Goiko successfully passed her MSC thesis exam. Masha has been a long-serving member of the Heit and deBruyn labs, and performed leading-edge research into super-resolution microscopy, protein diffusion and cellular force generation. Masha’s work has led to one submitted paper, and at least two additional papers are currently in-preparation. Masha …

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We Want You!

The Heit lab will have openings for one or two new Graduate Students to start in April 2014 or September 2014.  Applicants with a background in cell biology, biochemistry or immunology are preferred.  Prior research experience is strongly recommended. Applicants must: 1) Apply first to the Department of Microbiology and Immunology. Only applications from students …

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