Phagocyte Biology Laboratory

Dr. Bryan Heit, Western University

Computer Code


Molecular Interactions in Super Resolution (MIiSR) is our recently published super resolution image analysis package. This software package allows for the quantification of intermolecular interactions and the formation of higher-order molecular structures in super-resolution images. Downloads of the original version and new versions are available from this link.

Super-Resolution Image Analysis Scripts (Matlab)

Spatial Association Analysis (SAA): Matlab scripts used to analyze inter-molecular interactions within super-resolution images. Download includes scripts for assessing interactions between two and three sets of molecule, as well as files for converting particle position file formats and for identifying repeat detection of the same fluorophore. This package is replaced by MIiSR.

Random Matlab Code

Random code snipits used in the lab for various purposes. Code quality and documentation will not be upto the standard of code provided as part of publications. Use this code at your own risk!.

3D Printer Files

Replica plating block, for 10 cm (100 mm) petri dishes. Cloth needs to be held in place with an elastic. When printing, use multiple shells to ensure proper wall thickness. Recommend printing with PETG (Polyethylene terephthalate glycol) filament if using chloroform to sterilise.Polycarbonate is recommended if the cylinder needs to be autoclavable. File format: Zip-compressed .stl

Spaceships. Our custom Leiden chambers for use with live cell microscopy. These hold an 18 mm circular coverslip plus enough media to keep cells alive over 24 hours of imaging. The .ZIP file contains a readme file with all of the information required to print these chambers (including a complete list of non-3D printed parts), .STL files for two different size chambers (35 mm and 42 mm) and a .STL file for tools to aid in the assembly of the chambers. The only other item you will need is access to a 3D printer (we use the Prusa MK3S).