February 2018

Quantification of apoptotic cell uptake by 3D fluorescence microscopy

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This video shows an example of how we visualize and quantify efferocytosis. During efferocytosis, apoptotic (dying) cells are taken up piecemeal by the engulfing macrophage. TO visualize this process we pre-label the cytosol of the apoptotic cell with a protein reactive fluorophore such as Cell Proliferation Dye e670, and coat the surface of the apoptotic […]

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Quantifying Cholesterol Uptake by Macrophages

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In this short Youtube video we demonstrate a simple method of measuring cholesterol uptake and intracellular transport in macrophages. In this experiment, macrophages are cultured in a media containing oxLDL (bad cholesterol) and a cholesterol/lipid-reactive dye (Nile red). Live cell time-lapse microscopy is then used to monitor the accumulation of cholesterol by the cell. The

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Measuring Phagosomal pH

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We have published a brief Youtube video showing a phagosomal pH measurement experiment. This method can be used to assess phagosome, efferosome or endosome acidification using any target that can be conjugated to FITC, and using any cell type. The method automatically compensates for photobleaching and incorporates an in situ pH calibration to convert FITC ratios

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