Quantifying Cholesterol Uptake by Macrophages
In this short Youtube video we demonstrate a simple method of measuring cholesterol uptake and intracellular transport in macrophages. In this experiment, macrophages are cultured in a media containing oxLDL (bad cholesterol) and a cholesterol/lipid-reactive dye (Nile red). Live cell time-lapse microscopy is then used to monitor the accumulation of cholesterol by the cell. The […]
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