Front-Page Posts

Quantification of apoptotic cell uptake by 3D fluorescence microscopy

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This video shows an example of how we visualize and quantify efferocytosis. During efferocytosis, apoptotic (dying) cells are taken up piecemeal by the engulfing macrophage. TO visualize this process we pre-label the cytosol of the apoptotic cell with a protein reactive fluorophore such as Cell Proliferation Dye e670, and coat the surface of the apoptotic […]

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Measuring Phagosomal pH

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We have published a brief Youtube video showing a phagosomal pH measurement experiment. This method can be used to assess phagosome, efferosome or endosome acidification using any target that can be conjugated to FITC, and using any cell type. The method automatically compensates for photobleaching and incorporates an in situ pH calibration to convert FITC ratios

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Armed for Destruction – A new paper on Neutrophils

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The Heit lab is excited to announce the publication of our newest paper, a review on how neutrophil granules contribute to the potent antimicrobial capabilities of neutrophils. New paper published, on how neutrophils are armed to fight infection. Free read-only version available here: https://t.co/1K0WVSY2v2 pic.twitter.com/SzUDSQgRmx — Bryan Heit (@BryanHeit) November 29, 2017 A free read-only

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Two New Papers

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We are happy to have published two new papers with our collaborators over the past two weeks. The first paper was published with our collaborators Dr. Warren Lee and Dr. Greg Fairn, both from the St. Michael’s Hospital in Toronto, where we identified the mechanisms mediating the transport of HDL (good cholesterol) into the brain. Unlike

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